Table 2.

GO cellular processes for all phenotype categories. A summary of the GO analysis to identify genes annotated to cellular processes enriched within particular phenotype categories. The enrichment results were mapped to ‘GO slim’ (high level) terms covering most biological processes observed in fission yeast to give a broad view of the ontology content of the genome-wide gene deletion dataset. For details see §5.5.2 and the electronic supplementary material 1, table S6a–n and table S14. The total dataset is 4843 genes. Footnotes are denoted by a–n. Red colour denotes enriched p ≤ 0.001; orange denotes moderately enriched p ≤ 0.01; light orange denotes weakly enriched p ≤ 0.1: light blue denotes no enrichment p ≤ 1; blank denotes number of genes is 0.

Embedded Image
  • aIncludes 15/29 genes annotated to glycosylphosphatidylinositol (GPI) anchor biosynthesis, a descendent of lipid metabolism (p = 1.75 × 10−8).

  • bIncludes 10/41 genes annotated to attachment of spindle to microtubules, a descendent of chromosome segregation (p = 0.001).

  • cIncludes 11/26 genes annotated to histone deacetylation, a descendent of transcription (p = 0.00054).

  • dIncludes 53/118 genes annotated to nuclear mRNA splicing, via spliceosome, a descendent of mRNA metabolism (p = 3.7 × 10−27).

  • eIncludes 5/6 subunits of the elongator complex involved in tRNA wobble uridine modification.

  • fIncludes 11/25 genes annotated to the septation initiation signalling cascade (p = 0.00013), and 5/15 genes annotated to the stress activated protein. Kinase signalling cascade, 4/19 genes annotated to TOR signalling and 3/17 genes annotated to cAMP-mediated signalling (none enriched), all descendents of signalling.

  • gIncludes 27/79 genes annotated to regulation of interphase, a descendent of regulation of the mitotic cell cycle (p = 1.23 × 10−9).

  • hIncludes 18/67 genes annotated to regulation of mitosis (p = 4.88 × 10−11) and 6/27 genes annotated to attachment of spindle microtubules to kinetochore (p = 0.0351), descendents of regulation of the mitotic cell cycle.

  • iIncludes 13/115 genes annotated to microtubule cytoskeleton, a descendent of cytoskeleton organization (p = 0.00706).

  • jIncludes 4/9 genes annotated to Cdc42 signal transduction, a descendent of signalling (p = 0.006).

  • kIncludes 7/52 genes annotated to actin cytoskeleton organization, a descendent of cytoskeleton organization (p = 8.09 × 10−5).

  • lIncludes 13/115 genes annotated to microtubule cytoskeleton organization, a descendent of cytoskeleton organization (p = 2.12 × 10−8) and 5/5 genes annotated to gamma tubulin complex localization, a descendent of microtubule cytoskeleton organization (p = 3.11 × 10−8).

  • mIncludes 3/11 genes annotated to carbon catabolite repression of transcription, a descendent of signalling (p = 0.00484).

  • nAll 19 genes involved in mitochondrial tRNA metabolism.