- Figure S1 - Cell toxicity was measured by LDH release in neuron-like cells after 6, 16, 24h, 36h and 48 hours post transfected with GFP-DISC1. The bar graph represents the percentage of cytotoxicity normalized to control values. There were no significant differences between control and GFP-DISC1 transfected cells at any of the time points. (ns, not significant by two-tailed Mann Whitney test)
- Figure S2 - DISC1 large aggregates form aggresomes in neuronal cells
- Figure S3 - DISC1 small and medium aggregates are not confined to the endolysosomal pathway
- Figure S4 - GFP soluble protein does not transfer between cells. Representative confocal images of GFP-vector (Green) and H2B-mCherry transfected cells (Red, also with WGA556 to identify TNTs between cells). DAPI was used to stain nucleus (Blue).